Analytical Ultracentrifugation

Analytical Ultracentrifuges

Location: LFG, Haberstraße 9a, 91058 Erlangen, Room U1.826

Analytical ultracentrifugation is one of the most versatile and accurate methods for characterizing proteins, macromolecules, nanoparticles and other heterogeneous mixtures. Analytical ultracentrifugation provides direct access to the size, density and shape of particulate systems. The principle of sedimentation analysis is based on the optical detection of radius- and time-resolved signals. An important distinguishing feature of analytical ultracentrifuges is their detection systems.

M. Sc. Paola Cardenas (Optima AUC, Optima XL-90K with Multiwavelength Extinction Detector)

M. Sc. Hadi Soltanmoradi (Optima XL-80K with Multiwavelength Emission Detecor)

Analytical Ultracentrifuge | Beckman Coulter, Optima XL-90K with Multiwavelength Extinction Detector

Analytical ultracentrifuge with multi-wavelength extinction detector, particularly suitable for three-dimensional sedimentation analysis. Ability to extract sedimentation coefficient dependent UV-Vis-NIR spectra. For polydisperse samples, spectral assignment to different sizes/fractions is possible.

  • Rotor speed: 1,000 rpm to 60,000 rpm
  • Rotor: 4-hole (for up to 8 samples) or 8-hole (for up to 16 samples)
  • Sample volume: 0.1 mL to 0.4 mL
  • Particle size: 0.2 nm to 1000 nm (depending on particle density)
  • Detector: multiwavelength extinction detector (220 nm to 1,100 nm)
  • Temperature control: 4 to 40 °C
  • Measuring cell: various materials and optical path lengths
  • Measurement modes: sedimentation velocity, sedimentation equilibrium, speed ramp
Analytical Ultrazentrifuge | Beckman Coulter, Optima XL-80K with Multiwavelength Emission Detector

Analytical ultracentrifuge with multi-wavelength emission detector, particularly suitable for three-dimensional sedimentation analysis. Ability to extract sedimentation coefficient dependent emission spectra. This allows spectral assignment to different sedimentation coefficients/fractions for polydisperse samples. The instrument is equipped with lasers with excitation wavelengths of 405 nm and 520 nm.

  • Rotor speed: 1,000 rpm to 50,000 rpm
  • Rotor: 8-hole (for up to 16 samples)
  • Sample volume: 0.35 mL
  • Particle size: 0.2 nm to 1000 nm (depending on particle density)
  • Detector: multiwavelength emission detector (405 nm/520 nm to 1,100 nm)
  • Temperature control: 4 to 40 °C
  • Measuring cell: including 3 mm titanium centerpiece
  • Measurement modes: sedimentation velocity, sedimentation equilibrium, speed ramp
Analytical Ultracentrifuge | Beckman Coulter, Optima AUC

Analytical ultracentrifuge with single wavelength extinction detector and interference detector. The interference detector is particularly suitable for the analysis of non-absorbent and concentrated samples.

  • Rotor speed: 1,000 rpm to 60,000 rpm
  • Rotor: 4-hole (for up to 6 samples) or 8-hole (for up to 14 samples)
  • Sample volume: 0.1 mL to 0.4 mL
  • Particle size: 0.2 nm to 1000 nm (depending on particle density)
  • Detector: extinction (190 to 800 nm) and interference
  • Temperature control: 5 °C to 40 °C
  • Measuring cell: various materials and optical path lengths
  • Measurement modes: sedimentation velocity, sedimentation equilibrium, speed ramp

https://doi.org/10.1021/nn503205k

https://doi.org/10.1021/acs.analchem.7b04056

https://doi.org/10.1039/C9NA00487D

https://doi.org/10.1039/D3NA00980G

https://www.beckman.de/centrifuges/analytical-ultracentrifuges/optima-auc

Further information to follow